Continous-flow qPCR thermocycler for single tubes

Replicon presents a novel continuous-flow single tube qPCR instrument. Samples are taken every 7 seconds which are 500+ tests per hour. Typical time-to-result for a 38 cycle RT-qPCR is 22 min; the software allows detection of 100,000 copies target after ~15 min. The current prototype reads 2 dyes, which can be expanded to 4. The concept allows to break down multiplex PCR to successive PCR one after the other, to test exactly those targets requested, eliminating multiplex PCR validations.